MP08-01 BRAIN α7 NICOTINIC ACETYLCHOLINE RECEPTORS ARE INVOLVED IN SUPPRESSION OF THE RAT MICTURITION REFLEX VIA BRAIN GABA RECEPTORS

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology Pharmacology (MP08)1 Sep 2021MP08-01 BRAIN α7 NICOTINIC ACETYLCHOLINE RECEPTORS ARE INVOLVED IN SUPPRESSION OF THE RAT MICTURITION REFLEX VIA GABA Takahiro Shimizu, Yohei Suo Zou, Hideaki Ono, Yurika Hata, Masaki Yamamoto, Takaaki Aratake, Shogo Youichirou Higashi, Takashi Karashima, Masashi Honda, and Motoaki Saito ShimizuTakahiro Shimizu More articles by this author , ShimizuYohei ZouSuo Zou OnoHideaki Ono HataYurika Hata YamamotoMasaki Yamamoto AratakeTakaaki Aratake ShimizuShogo HigashiYouichirou Higashi KarashimaTakashi Karashima HondaMasashi Honda SaitoMotoaki View All Author Informationhttps://doi.org/10.1097/JU.0000000000001981.01AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Brain nicotinic acetylcholine receptors (nAChRs) are reported suppress the micturition reflex, but brain mechanisms for suppression unclear. In study, we examined centrally administered epibatidine (EP, non-selective nAChR agonist)-induced effects on focusing representative subtypes (α4β2 α7) in rats. METHODS: Urethane anesthetized (0.8 g/kg, ip) male Wistar rats (300-450 g) were used. A catheter was inserted into bladder perform cystometry (12 ml/h saline infusion). Three hours after surgery, EP (0.3 or 1 nmol/rat) vehicle icv administered. some rats, pretreated mecamylamine (MEC, antagonist, 100 300 nmol/rat), methyllycaconitine (MLA, selective 30 dihydro-β-erythroidine (DHβE, α4β2 SR95531 (SR, GABAA 0.03 0.1 SCH50911 (SCH, GABAB (1 nmol/rat, icv)-induced responses examined. Effects PHA568487 (PHA, agonist, 0.3 icv) urodynamic parameters also Cystometry evaluation intercontraction intervals (ICI) maximal voiding pressure (MVP) started 60 min before first administration. RESULTS: dose-dependently prolonged ICI without changing MVP (table 1). The EP-induced prolongation suppressed MEC, MLA, SR SCH, not DHβE 2-3). PHA 4), similar EP. CONCLUSIONS: nAChRs involved rat reflex via receptors, therefore, might be new therapeutic targets neurogenic overactivity. Source Funding: Smoking Research Foundation Japan, JSPS KAKENHI (#17K09303, #20K07827), Takeda Science © 2021 American Urological Association Education Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e149-e149 Advertisement Copyright Permissions© Inc.MetricsAuthor Information Expand Loading ...